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slot blot apparatus  (Hoefer)

 
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    Hoefer slot blot apparatus
    Slot Blot Apparatus, supplied by Hoefer, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slot+blot+apparatus/us12599655-883-27-29?v=Hoefer
    Average 86 stars, based on 1 article reviews
    slot blot apparatus - by Bioz Stars, 2026-06
    86/100 stars

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    A slot blot apparatus placed on a raised surface (e.g., an upside-down 1 L plastic beaker) and attached to a vacuum line with a three-way stopcock air valve.

    Journal: Bio-protocol

    Article Title: Selective Isolation of TOP3B•mRNA Covalent Intermediates Using Denaturing Oligo-dT Pulldown

    doi: 10.21769/BioProtoc.5627

    Figure Lengend Snippet: A slot blot apparatus placed on a raised surface (e.g., an upside-down 1 L plastic beaker) and attached to a vacuum line with a three-way stopcock air valve.

    Article Snippet: If using the Bio-Rad slot blot apparatus, two pieces of filter paper (equilibrated in 20 mM Tris-HCl) should be added below the nylon and nitrocellulose membranes ( ).

    Techniques: Dot Blot

    Onto the bottom piece of the slot blot apparatus, two pieces of filter paper should be placed only when using the Bio-Dot apparatus . For all slot blots, two pieces of positively charged nylon membrane, followed by 1 nitrocellulose membrane, should be placed before securing the top slot blot piece in place.

    Journal: Bio-protocol

    Article Title: Selective Isolation of TOP3B•mRNA Covalent Intermediates Using Denaturing Oligo-dT Pulldown

    doi: 10.21769/BioProtoc.5627

    Figure Lengend Snippet: Onto the bottom piece of the slot blot apparatus, two pieces of filter paper should be placed only when using the Bio-Dot apparatus . For all slot blots, two pieces of positively charged nylon membrane, followed by 1 nitrocellulose membrane, should be placed before securing the top slot blot piece in place.

    Article Snippet: If using the Bio-Rad slot blot apparatus, two pieces of filter paper (equilibrated in 20 mM Tris-HCl) should be added below the nylon and nitrocellulose membranes ( ).

    Techniques: Dot Blot, Membrane